Device

Part:BBa_K4407015:Design

Designed by: Leyu Xu   Group: iGEM22_Worldshaper-Nanjing   (2022-10-03)


Pthl-dps-aceE，Coexpression of dps and aceE with Pthl promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2349
    Illegal BglII site found at 2757
    Illegal BamHI site found at 1309
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3379
    Illegal AgeI site found at 2090
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In our project, we used this device to construct facultative anaerobic Clostridium tyrobutyricum (C. tyrobutyricum). dps protects the bacteria from oxidative damage. It protects DNA against oxidative damage by binding with Fe2+ to prevent it from reacting with H2O2 (Fenton’s reaction). aceE protein is a component of the PDH complex and catalyzes the conversion of pyruvate to acetyl-CoA and CO2. It can function well in aerobic environment. This device combines the function of the two proteins, enhancing viability in aerobic environment by protecting DNA and substituting the main metabolic enzyme PFOR which is inactivated under aerobic condition with PDH in the presence of oxygen.


Source

Pthl is based on BBa_K3443002. dps is based on BBa_K4407001. aceE is based on BBa_K4407002. Terminator is based on BBa_K3585002.

References